{"id":6289,"date":"2024-04-03T18:25:30","date_gmt":"2024-04-04T00:25:30","guid":{"rendered":"https:\/\/meximagingworkshops.org.mx\/?page_id=6289"},"modified":"2024-04-15T15:13:05","modified_gmt":"2024-04-15T20:13:05","slug":"mbw8-lightsheet-microscopy-tissue-clearing-and-expansion","status":"publish","type":"page","link":"https:\/\/meximagingworkshops.org.mx\/es\/mbw8-lightsheet-microscopy-tissue-clearing-and-expansion\/","title":{"rendered":"MBW8: Lightsheet Microscopy, Tissue Clearing and Expansion"},"content":{"rendered":"
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Summary:\u00a0<\/strong><\/p>

Lightsheet microscopy, also known as selective plane illumination microscopy (SPIM), is a fluorescence microscopy technique that combines optical sectioning with imaging from multiple views. This facilitates the observation of biological samples and organisms under the principle of tomography for both translucent and opaque samples at different depths. The difference with widefield fluorescence microscopy or confocal microscopy is that lightsheet microscopy only illuminates one optical section at a time, minimizing the effects of photobleaching and phototoxicity. Additionally, the use of computational algorithms allows for three-dimensional reconstruction, even at different time points, generating either a static image or a 3D video as required. The application of this microscopy technique is broad because it can visualize a wide variety of specimens, such as embryos, organoids, spheroids, small animals, small organs, insects, plants, biopsies, etc., which can be cleared or expanded to increase either the depth of view or the resolution.<\/p>

This is a theoretical-demonstrative course with two main objectives. The first is to introduce lightsheet microscopy; sample preparation through clearing or expansion; and image data management and processing. The second objective is to foster the formation of a regional community of lightsheet microscopy imaging scientists focused on solving scientific and medical problems through the study of the structure and dynamics of three-dimensional biological models that cannot be seen by other microscopy techniques.<\/p><\/div>\n \t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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MBW8: Lightsheet Microscopy, Tissue Clearing and Expansion – Day 1<\/div>\n
May 6, 2024<\/div>\n <\/div>\n <\/div>\n
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Registro<\/h3>\n
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\n <\/i><\/span>\n \n By <\/span> Alejandro L\u00f3pez<\/a> <\/span>\n <\/div>\n <\/div>\n\n
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Bienvenida<\/h3>\n
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Alejandro L\u00f3pez<\/div>\n
INCAN<\/div>\n <\/div>\n <\/div>\n <\/div>\n

Alejandro L\u00f3pez Saavedra (INCan), M\u00f3nica Ramirez (FacMed, UNAM)<\/p>\n<\/div>\n

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\n <\/i><\/span>\n \n By <\/span> Diego Delgado (LNMA-CICESE)<\/a> <\/span>\n <\/div>\n <\/div>\n\n
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Connecting the Mexican Bioimaging Community<\/h3>\n
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Diego Delgado (LNMA-CICESE)<\/div>\n
LNMA-CICESE<\/div>\n <\/div>\n <\/div>\n <\/div>\n

Diego Delgado \u00c1lvarez (LNMA-CICESE)<\/p>\n<\/div>\n

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\n <\/i><\/span>\n \n By <\/span> Jos\u00e9 Manuel Mart\u00ednez L\u00f3pez (Quimica TECH)<\/a> <\/span>\n <\/div>\n <\/div>\n\n
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Microscop\u00eda de Fluorescencia<\/h3>\n
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Jos\u00e9 Manuel Mart\u00ednez L\u00f3pez (Quimica TECH)<\/div>\n
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Jos\u00e9 Manuel Mart\u00ednez L\u00f3pez (Qu\u00edmica Tech)<\/p>\n<\/div>\n

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\n <\/i><\/span>\n \n By <\/span> Miguel Tapia (Instituo de Investigaciones Biom\u00e9dicas)<\/a> <\/span>\n <\/div>\n <\/div>\n\n
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Microscop\u00eda de seccionamiento \u00f3ptico<\/h3>\n
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Miguel Tapia (Instituo de Investigaciones Biom\u00e9dicas)<\/div>\n
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Miguel Tapia Rodr\u00edguez (IIB, UNAM)<\/p>\n<\/div>\n

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\n <\/i><\/span>\n \n By <\/span> Pablo Loza<\/a> <\/span>\n <\/div>\n <\/div>\n\n
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Conceptos b\u00e1sicos en la microscop\u00eda de Hoja de Luz<\/h3>\n
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Pablo Loza<\/div>\n
ICFO<\/div>\n <\/div>\n <\/div>\n <\/div>\n

Pablo Loza \u00c1lvarez (SLN, ICFO, Barcelona)<\/p>\n<\/div>\n

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Coffee- break<\/h3>\n
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\n <\/i><\/span>\n \n By <\/span> Ruth Rinc\u00f3n Heredia<\/a> <\/span>\n <\/div>\n <\/div>\n\n
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Preparaci\u00f3n de muestras biol\u00f3gicas para su observaci\u00f3n al microscopio de fluorescencia<\/h3>\n
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Ruth Rinc\u00f3n Heredia<\/div>\n
Instituto de Fisiolog\u00eda Celular (UNAM).<\/div>\n <\/div>\n <\/div>\n <\/div>\n

Ruth Rinc\u00f3n Heredia (IFC, UNAM)<\/p>\n<\/div>\n

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Expansi\u00f3n de tejidos y microscop\u00eda de hoja de luz: explorando la nanoestructura de organoides<\/h3>\n

Juan E. Rodr\u00edguez Gatica (Clausius Institut f\u00fcr Physikalische und Theoritische Chemie)<\/p>\n<\/div>\n <\/div>\n <\/div>\n <\/div>\n

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Sesi\u00f3n de rompehielo y formaci\u00f3n de grupos de trabajo<\/h3>\n
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Comida<\/h3>\n
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Sesiones de pr\u00e1ctica-demo - Rotaci\u00f3n de grupos de trabajo<\/h3>\n

Unidad de Aplicaciones Avanzadas en Microscop\u00eda (ADMiRA), INCan<\/p>\n<\/div>\n <\/div>\n <\/div>\n <\/div>\n

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Coffee- break<\/h3>\n
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Sesiones de pr\u00e1ctica-demo - Rotaci\u00f3n de grupos de trabajo<\/h3>\n

Unidad de Aplicaciones Avanzadas en Microscop\u00eda (ADMiRA), INCan<\/p>\n<\/div>\n <\/div>\n <\/div>\n <\/div>\n

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Regreso al Hotel Centro Cultural Quinta Soledad<\/h3>\n
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MBW8: Lightsheet Microscopy, Tissue Clearing and Expansion – Day 2<\/div>\n
May 7, 2024<\/div>\n <\/div>\n <\/div>\n
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\n <\/i><\/span>\n \n By <\/span> Lia Pietrasanta (LABI)<\/a> <\/span>\n <\/div>\n <\/div>\n\n
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Presentaci\u00f3n de la red Latin America Bioimaging (LABI) (virtual)<\/h3>\n
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Lia Pietrasanta (LABI)<\/div>\n
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L\u00eda Pietrasanta (Universidad de Buenos Aires)<\/p>\n<\/div>\n

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\n <\/i><\/span>\n \n By <\/span> Jacques Paysan (Carl Zeiss)<\/a> <\/span>\n <\/div>\n <\/div>\n\n
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The art of clearing<\/h3>\n
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Jacques Paysan (Carl Zeiss)<\/div>\n
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The art of clearing<\/p>\n<\/div>\n

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Aclarado \u00f3ptico de tejidos<\/h3>\n

Yoloxochitl S\u00e1nchez Guevara (IBT, UNAM)<\/p>\n<\/div>\n <\/div>\n <\/div>\n <\/div>\n

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\n <\/i><\/span>\n \n By <\/span> Alejandro Olvera Ordo\u00f1ez (Carl Zeiss)<\/a> <\/span>\n <\/div>\n <\/div>\n\n
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Im\u00e1genes digitales y selecci\u00f3n de c\u00e1maras<\/h3>\n
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Alejandro Olvera Ordo\u00f1ez (Carl Zeiss)<\/div>\n
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Im\u00e1genes digitales y selecci\u00f3n de c\u00e1maras<\/p>\n<\/div>\n

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Coffee Break<\/h3>\n
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\n <\/i><\/span>\n \n By <\/span> Tomas Silva Santisteban (BitPlane, Oxford)<\/a> <\/span>\n <\/div>\n <\/div>\n\n
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Imaris: An\u00e1lisis de im\u00e1genes de microscop\u00eda utilizando inteligencia artificial para todos<\/h3>\n
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Tomas Silva Santisteban (BitPlane, Oxford)<\/div>\n
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Tomas Silva Santisteban (BitPlane, Oxford)<\/p>\n<\/div>\n

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Scalable image analysis of multidimensional lightsheet data with Zeiss Arivis (virtual)<\/h3>\n

Molly McQuilken (Carl Zeiss)<\/p>\n<\/div>\n <\/div>\n <\/div>\n <\/div>\n

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\n <\/i><\/span>\n \n By <\/span> Mauricio D. Cerda Villablanca (Universidad de Chile)<\/a> <\/span>\n <\/div>\n <\/div>\n\n
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Procesamiento de im\u00e1genes lightsheet con FIJI: registro, visualizaci\u00f3n masiva, y cuantificaci\u00f3n<\/h3>\n
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Mauricio D. Cerda Villablanca (Universidad de Chile)<\/div>\n
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Mauricio D. Cerda Villablanca (Universidad de Chile)<\/p>\n<\/div>\n

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Comida<\/h3>\n
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Sesiones de pr\u00e1ctica-demo - Rotaci\u00f3n de grupos de trabajo<\/h3>\n

Unidad de Aplicaciones Avanzadas en Microscop\u00eda (ADMiRA), INCan<\/p>\n<\/div>\n <\/div>\n <\/div>\n <\/div>\n

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Coffee break<\/h3>\n
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Sesiones de pr\u00e1ctica-demo - Rotaci\u00f3n de grupos de trabajo<\/h3>\n

Unidad de Aplicaciones Avanzadas en Microscop\u00eda (ADMiRA), INCan<\/p>\n<\/div>\n <\/div>\n <\/div>\n <\/div>\n

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Regreso al Hotel Centro Cultural Quinta Soledad<\/h3>\n
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MBW8: Lightsheet Microscopy, Tissue Clearing and Expansion – Day 3<\/div>\n
May 8, 2024<\/div>\n <\/div>\n <\/div>\n
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\n <\/i><\/span>\n \n 8:00 am - 8:40 am <\/span>\n <\/div>\n
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\n <\/i><\/span>\n \n By <\/span> Israel Rocha Mendoza (CICESE)<\/a> <\/span>\n <\/div>\n <\/div>\n\n
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Microscop\u00eda fluorescente con hojas de luz l\u00e1ser: ventajas, desaf\u00edos y colaboraciones en su implementaci\u00f3n<\/h3>\n
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Israel Rocha Mendoza (CICESE)<\/div>\n
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Israel Rocha Mendoza (CICESE)<\/p>\n<\/div>\n

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\n <\/i><\/span>\n \n By <\/span> Pablo Loza<\/a> <\/span>\n <\/div>\n <\/div>\n\n
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Visualizaci\u00f3n volum\u00e9trica r\u00e1pida con LSFM y aplicaciones a la biomedicina<\/h3>\n
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Pablo Loza<\/div>\n
ICFO<\/div>\n <\/div>\n <\/div>\n <\/div>\n

Pablo Loza \u00c1lvarez (SLN, ICFO, Barcelona)<\/p>\n<\/div>\n

<\/i><\/div>\n <\/div>\n <\/div>\n <\/div>\n
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\n <\/i><\/span>\n \n By <\/span> J. Eduardo Rodr\u00edguez<\/a> <\/span>\n <\/div>\n <\/div>\n\n
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Desentra\u00f1ando redes neuronales: Iluminando conexiones en cerebros de rat\u00f3n expandidos.<\/h3>\n
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J. Eduardo Rodr\u00edguez<\/div>\n
<\/div>\n <\/div>\n <\/div>\n <\/div>\n

Juan E. Rodr\u00edguez Gatica (Clausius Institut f\u00fcr Physikalische und Theoritische Chemie)<\/p>\n<\/div>\n

<\/i><\/div>\n <\/div>\n <\/div>\n <\/div>\n
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\n <\/i><\/span>\n \n By <\/span> Jacques Paysan (Carl Zeiss)<\/a> <\/span>\n <\/div>\n <\/div>\n\n
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The art of sample mounting<\/h3>\n
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Jacques Paysan (Carl Zeiss)<\/div>\n
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Coffee- break<\/h3>\n
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Sesiones de pr\u00e1ctica-demo - Rotaci\u00f3n de grupos de trabajo<\/h3>\n
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Comida<\/h3>\n
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Sesiones de pr\u00e1ctica-demo - Rotaci\u00f3n de grupos de trabajo<\/h3>\n

Unidad de Aplicaciones Avanzadas en Microscop\u00eda (ADMiRA), INCan<\/p>\n<\/div>\n <\/div>\n <\/div>\n <\/div>\n

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Sesiones de pr\u00e1ctica-demo - Rotaci\u00f3n de grupos de trabajo<\/h3>\n

Unidad de Aplicaciones Avanzadas en Microscop\u00eda (ADMiRA), INCan<\/p>\n<\/div>\n <\/div>\n <\/div>\n <\/div>\n

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Coffee- break<\/h3>\n
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Sesiones de pr\u00e1ctica-demo - Rotaci\u00f3n de grupos de trabajo<\/h3>\n

Unidad de Aplicaciones Avanzadas en Microscop\u00eda (ADMiRA), INCan<\/p>\n<\/div>\n <\/div>\n <\/div>\n <\/div>\n

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Regreso al Hotel Centro Cultural Quinta Soledad<\/h3>\n
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CENA<\/h3>\n
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MBW8: Lightsheet Microscopy, Tissue Clearing and Expansion – Day 4<\/div>\n
May 9, 2024<\/div>\n <\/div>\n <\/div>\n
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\n <\/i><\/span>\n \n 8:15 am - 8:55 am <\/span>\n <\/div>\n
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\n <\/i><\/span>\n \n By <\/span> Julio Ch\u00e1vez Zamora (IBT, UNAM)<\/a> <\/span>\n <\/div>\n <\/div>\n\n
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Estudio de la migraci\u00f3n de espermatozoides de rat\u00f3n dentro del tracto reproductor femenino mediante aclarado \u00f3ptico de tejidos<\/h3>\n
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Julio Ch\u00e1vez Zamora (IBT, UNAM)<\/div>\n
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Presentation of the Bioimaging North America (BINA) network (virtual)<\/h3>\n
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Nikki Bialy<\/div>\n
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Nikki Bialy (Morgridge Institute for Research)<\/p>\n<\/div>\n

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\n <\/i><\/span>\n \n By <\/span> Jos\u00e9 Manuel Mart\u00ednez L\u00f3pez (Quimica TECH)<\/a> <\/span>\n <\/div>\n <\/div>\n\n
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Arte y Microscop\u00eda<\/h3>\n
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Jos\u00e9 Manuel Mart\u00ednez L\u00f3pez (Quimica TECH)<\/div>\n
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Jos\u00e9 Manuel Mart\u00ednez L\u00f3pez (Qu\u00edmica Tech)<\/p>\n<\/div>\n

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Clausura<\/h3>\n
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Foto grupal<\/h3>\n
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Coffee- break<\/h3>\n
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\n <\/i><\/span>\n \n 10:40 am - 10:40 am <\/span>\n <\/div>\n
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Transporte a Centro de Tlalpan para evento de divulgaci\u00f3n<\/h3>\n
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Evento de divulgaci\u00f3n<\/h3>\n

Plaza del Bolero \u201cArmando Manzanero\u201d | Centro de Tlalpan<\/p>\n<\/div>\n <\/div>\n <\/div>\n <\/div>\n <\/div>\n <\/div>\n <\/div>\n <\/div>\n <\/div>\n \t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"

Summary:\u00a0 Lightsheet microscopy, also known as selective plane illumination microscopy (SPIM), is a fluorescence microscopy technique that combines optical sectioning with imaging from multiple views. This facilitates the observation of biological samples and organisms under the principle of tomography for both translucent and opaque samples at different depths. The difference with widefield fluorescence microscopy or … Read More “Day 1”<\/span><\/a><\/span><\/p>","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"om_disable_all_campaigns":false,"inline_featured_image":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"class_list":["post-6289","page","type-page","status-publish","hentry"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/meximagingworkshops.org.mx\/es\/wp-json\/wp\/v2\/pages\/6289","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/meximagingworkshops.org.mx\/es\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/meximagingworkshops.org.mx\/es\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/meximagingworkshops.org.mx\/es\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/meximagingworkshops.org.mx\/es\/wp-json\/wp\/v2\/comments?post=6289"}],"version-history":[{"count":42,"href":"https:\/\/meximagingworkshops.org.mx\/es\/wp-json\/wp\/v2\/pages\/6289\/revisions"}],"predecessor-version":[{"id":6415,"href":"https:\/\/meximagingworkshops.org.mx\/es\/wp-json\/wp\/v2\/pages\/6289\/revisions\/6415"}],"wp:attachment":[{"href":"https:\/\/meximagingworkshops.org.mx\/es\/wp-json\/wp\/v2\/media?parent=6289"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}