Hands-on workshop on super-resolution microscopy
October 6, 2022

Welcome at LNMA-IBt

Adán Guerrero Cárdenas (LNMA-UNAM)
Instituto de Biotecnología (UNAM)
3:15 pm - 3:25 pm

Social perception questionnaire

Roberto López

Tour at LNMA-IBt. (TIRF, Nanoimager, Confocal)

David Torres (LNMA-UNAM)
Diana Vázquez (LNMA-UNAM)
5:45 pm - 6:00 pm

-

6:00 pm - 8:00 pm

Transfer to Hotel Montetaxco

Hands-on workshop on super-resolution microscopy – Day 1
October 7, 2022
8:30 am - 8:35 am
Hotel Montetaxco

Welcome & Opening

Adán Guerrero Cárdenas (LNMA-UNAM)
Instituto de Biotecnología (UNAM)
8:35 am - 8:45 am
Hotel Montetaxco

Fluorescence Nanoscopy Bioimaging

Mariano Buffone (IBYME-CONICET)
IBYME-CONICET
8:45 am - 9:00 am
Hotel Montetaxco

Connecting the Mexican Bioimaging Community

Diego Delgado (LNMA-CICESE)
LNMA-CICESE
9:00 am - 9:10 am
Hotel Montetaxco

Q&A

9:10 am - 9:30 am
Hotel Montetaxco

Breaking space: Mingle

Janaina García (INECOL)
9:30 am - 9:45 am
Hotel Montetaxco

Projects presentation G1

Janaina García (INECOL)
9:45 am - 10:00 am

Coffee Break

10:00 am - 10:30 am
Hotel Montetaxco

From the microscope to the PSF

Miguel Tapia (Instituo de Investigaciones Biomédicas)
10:30 am - 10:55 am
Hotel Montetaxco

Super-Resolution Microscopy: opening a new era

Haydee Hernández (LNMA-UNAM)
10:55 am - 11:00 am

Q & A

11:00 am - 11:30 am
Hotel Montetaxco

SIM, 4Pi, Lattice LIght Sheet, STED

Adán Guerrero Cárdenas (LNMA-UNAM)
Instituto de Biotecnología (UNAM)
11:30 am - 11:40 am

Break

11:40 am - 12:05 pm
Hotel Montetaxco

Scientific Talk: Super-resolution imaging reveals the structure disruption of Chromosome Territories 9 and 22 associated with treatment resistance in CML

Eunice Fabian-Morales (INCAN)
INCAN
12:05 am - 12:30 pm
Hotel Montetaxco

Scientific Talk: Over-elongation of centrioles in cancer promotes centriole amplification and chromosome missegregation

Adán Guerrero Cárdenas (LNMA-UNAM)
Instituto de Biotecnología (UNAM)
12:30 am - 12:40 pm

Q&A

12:40 pm - 1:00 pm
Hotel Montetaxco

Projects presentation G2

Janaina García (INECOL)
1:00 pm - 1:15 pm
Hotel Montetaxco

Projects presentation G3

Janaina García (INECOL)
1:15 pm - 1:30 pm

Q&A

1:30 pm - 3:00 pm

Lunch

3:00 pm - 3:30 pm
Hotel Montetaxco

Single Molecule Localization Microscopy (STORM/PALM)

Miguel Tapia (Instituo de Investigaciones Biomédicas)
3:30 pm - 3:35 pm

Q&A

3:35 pm - 4:45 pm
Hotel Montetaxco

ONI-Nanoimager (STORM 3D, SPT, smFRET)

4:45 pm - 5:00 pm

Q&A

Hands-on workshop on super-resolution microscopy – Day 2
October 8, 2022
8:30 am - 9:00 am
Hotel Montetaxco

Virtual demo - Single Molecule Localization Microscopy

Diana Vázquez (LNMA-UNAM)
9:00 am - 9:20 am
Hotel Montetaxco

Group Photo

9:20 am - 9:35 am
Hotel Montetaxco

DNA Paint

Eduardo Brito-Alarcón (IBT – UNAM)
9:35 am - 9:50 am
Hotel Montetaxco

Spatial Statistics

David Torres (LNMA-UNAM)
9:50 am - 9:55 am

Q&A

9:55 am - 10:15 am

Coffee Break

10:15 am - 10:20 am
Hotel Montetaxco

Fluorescence fluctuation based super resolution microscopy

Adán Guerrero Cárdenas (LNMA-UNAM)
Instituto de Biotecnología (UNAM)
10:20 am - 10:30 am
Hotel Montetaxco

Stochastic Optical Fluctuation Imaging: theory and applications

Eduardo Brito-Alarcón (IBT – UNAM)
10:30 am - 10:35 am

Q&A

10:35 am - 10:45 am
Hotel Montetaxco

Bayesian analysis of Blinking and Bleaching (3B) microscopy: theory and applications

Haydee Hernández (LNMA-UNAM)
10:45 am - 10:50 am
Hotel Montetaxco

Super Resolution Radial Fluctuation

David Torres (LNMA-UNAM)
10:50 am - 10:55 am

Q&A

10:55 am - 11:15 am
Hotel Montetaxco

Round table 1

Eunice Fabian-Morales (INCAN)
INCAN

Moderator: Eunice Fabián

Q1: FF-SRM techniques are accesible and easy to use on the day-to-day basis?
Q2: How is it possible to measure or detect artifacts in FF-SRM?

Expert 1. David Torres
Expert 2. Eduardo Brito

11:15 am - 11:35 am
Hotel Montetaxco

Round table 2

Mariano Buffone (IBYME-CONICET)
IBYME-CONICET

Moderator: Mariano Buffone

Q1: What computing resources are required for SRM?
Q2: Is it possible to do SRM in 3D? Why?

Expert 3. Raul Pinto
Expert 4. Haydee Hernández

11:35 am - 11:45 am
Hotel Montetaxco

Interaction Session about the plenary

11:45 am - 11:50 am

Break

11:50 am - 12:00 pm
Hotel Montetaxco

Talk: Nanoscale organization of viral replication machineries

Adán Guerrero Cárdenas (LNMA-UNAM)
Instituto de Biotecnología (UNAM)
12:00 pm - 12:25 pm
Hotel Montetaxco

Talk: SRM on Heterologous calcium-dependent inactivation of Orai1 by neighboring TRPV1 channels

Carlos Bastián (Nikon)
12:25 pm - 12:30 pm

Q&A

12:30 pm - 12:35 pm

Break

12:35 pm - 1:00 pm
Hotel Montetaxco

Mean-Shift Super-Resolution theory

Esley Torres (CIC-UAEM)
Raúl Pinto (IICBA – UAEM)
1:00 pm - 1:30 pm
Hotel Montetaxco

Mean-Shift Super-Resolution applications

Adán Guerrero Cárdenas (LNMA-UNAM)
Instituto de Biotecnología (UNAM)
1:25 pm - 1:30 pm

Q&A

1:30 pm - 3:00 pm

Lunch

3:00 pm - 3:35 pm
Hotel Montetaxco

Hands-On: First steps to use MSSR

3:35 pm - 3:40 pm

Q&A

3:40 pm - 4:05 pm

Hands-On: Generation of SRM with MSSR

4:05 pm - 4:45 pm
Hotel Montetaxco

Plenary Talk: Super resolution microscopy reveals dynamic changes in the mouse sperm midpiece

Mariano Buffone (IBYME-CONICET)
IBYME-CONICET
4:45 pm - 5:00 pm

Q&A

Hands-on workshop on super-resolution microscopy – Day 3
October 9, 2022
8:30 am - 11:30 am
Hotel Montetaxco

Hands-On: Generation of SRM with MSSR

11:30 am - 11:45 am

Coffee break

11:45 am - 12:35 pm
Hotel Montetaxco

Block 1: Student’s project presentations

12:35 pm - 12:45 pm

Q&A

12:45 pm - 1:20 pm

Block 2: Student’s project presentations

1:20 pm - 1:30 pm

Q&A

Hands-on workshop on super-resolution microscopy – Last day
October 10, 2022

Ceremony award and closing event

Mariano Buffone (IBYME-CONICET)
IBYME-CONICET
Adán Guerrero Cárdenas (LNMA-UNAM)
Instituto de Biotecnología (UNAM)
10:00 am - 10:30 am
Hotel Montetaxco

Final thoughts and feedback from assistants

11:30 am - 1:30 pm

Transfer to LNMA-IBt

Fundamentos de Microscopía óptica – Día 1
October 13, 2022
8:30 am - 8:45 am
CICY. Yucatán, México

Bienvenida e inauguración

Ileana Echevarría (Directora de la UBBMP, CICY)
Directora de la UBBMP, CICY
8:45 pm - 9:00 am
CICY. Yucatán, México

Presentación del taller CZI mexican Bioimaging y video

Diego Delgado (LNMA-CICESE)
LNMA-CICESE
9:00 am - 10:00 am
CICY. Yucatán, México

Conectando a los asistentes

Ángela Kú (CICY)
CICY
Diego Delgado (LNMA-CICESE)
LNMA-CICESE
10:00 am - 10:15 am

Café

10:15 am - 10:45 am
CICY. Yucatán, México

Viaje al centro de la luz

José Ramón Hernández (Museo de la Luz)
Museo de la Luz
10:45 am - 12:00 pm
CICY. Yucatán, México

Introducción al Manejo del Microscopio: Fundamentos de la óptica y mecánica del microscopio de luz e iluminación Köhler

Lino Sánchez
Cinvestav Irapuato
11:15 am - 11:45 am
CICY. Yucatán, México

Métodos de Contraste: Contraste de fase, Contraste diferencial de interferencia (DIC), Campo oscuro. Microscopia de campo claro

Adán Guerrero Cárdenas (LNMA-UNAM)
Instituto de Biotecnología (UNAM)
11:45 am - 12:00 pm

Café

Eunice Fabian-Morales (INCAN)
INCAN
12:00 pm - 12:30 pm
CICY. Yucatán, México

Cámaras y sensores

Diego Delgado (LNMA-CICESE)
LNMA-CICESE
12:30 pm - 1:00 pm
CICY. Yucatán, México

Aspectos generales sobre selección y preparación de muestras vegetales

Ángela Kú (CICY)
CICY
1:00 pm - 1:30 pm
CICY. Yucatán, México

Introducción a la Microscopía Confocal

Rosa Mouriño (LNMA-CICESE)
LNMA-CICESE
1:30 pm - 2:00 pm
CICY. Yucatán, México

¿Para qué sirve la impresión 3D? , divulgación de la ciencia

Pablo Rosi (Universidad de Buenos Aires)
Universidad de Buenos Aires
2:00 pm - 3:00 pm
CICY. Yucatán, México

Foto grupal, comida

3:00 pm - 5:00 pm
CICY. Yucatán, México

Sesión práctica demostrativa, histología e iluminación Kohler

Ángela Kú (CICY)
CICY
8:00 pm - 10:00 pm
CICY. Yucatán, México

Cena grupal

Fundamentos de Microscopía óptica – Día 2
October 14, 2022
8:30 am - 9:00 am
CICY. Yucatán, México

Microscopía de fluorescencia

Enrique Castaño
CICY
9:00 am - 9:30 am
CICY. Yucatán, México

Introducción a la Microscopía de Superresolución

Mariano Buffone (IBYME-CONICET)
IBYME-CONICET
9:30 am - 10:00 am

Café

10:00 am - 10:30 am
CICY. Yucatán, México

Introducción a la edición y análisis de imágenes

Adán Guerrero Cárdenas (LNMA-UNAM)
Instituto de Biotecnología (UNAM)
10:30 am - 11:00 am
CICY. Yucatán, México

Microscopia SIM bases y ejemplos

Eunice Fabian-Morales (INCAN)
INCAN
11:00 am - 11:30 am

Café

11:30 am - 12:00 pm
CICY. Yucatán, México

Evaluación de la citocompatibilidad de biomateriales mediante microscopía confocal

Nayelli Rodríguez
Biomateriales (CICY)
12:00 pm - 12:30 pm
CICY. Yucatán, México

Fulgencio Alatorre (CICY)
(CICY)
12:30 pm - 1:00 pm
CICY. Yucatán, México

Microscopía digital

Bruno Pinhate Do Carmo (Leica)
1:00 pm - 1:30 pm
CICY. Yucatán, México

Últimos avances en formación de imágenes automatizadas en microscopía con APX100

Murillo Pimentel (Evident Scientific/Olympus)
1:30 pm - 1:45 pm
CICY. Yucatán, México

BINA

Vanessa Orr
1:45 pm - 3:00 pm

Comida

3:00 pm - 5:00 pm
CICY. Yucatán, México

Sesión demostrativa de Microscopía Confocal

Ángela Kú (CICY)
CICY
Fundamentos de Microscopía óptica – Día 3
October 15, 2022

Evento de Apropiación social del conocimiento

Janaina García (INECOL)
Eunice Fabian-Morales (INCAN)
INCAN
Mariano Buffone (IBYME-CONICET)
IBYME-CONICET
Ángela Kú (CICY)
CICY
Diego Delgado (LNMA-CICESE)
LNMA-CICESE
Adán Guerrero Cárdenas (LNMA-UNAM)
Instituto de Biotecnología (UNAM)

Mesa 1: La microscopía en ciencias marinas, coordina Janaina y Adán
Mesa 2: Los hongos, coordina Diego
Mesa 3: Las plantas y sus texturas microscópicas. Coordina Ángela
Mesa 4: Las células animales, coordina Mariano y Eunice
Mesa 5: Cuarto de Fluorescencia. Coordina Angela

program outline

The specific dates of the events may vary.

Advanced Microscopy: Initiation to Advanced Microscopy

When

April-June 2022

Where

Science Faculty (UNAM).
Mexico City, Mexico

program

Coming soon

Image analysis: Fundamentals and applications on fluorescence microscopy

When

April-June 2022

Where

LNMA-UNAM Cuernavaca, Mexico​

program

Coming soon

Fundamentals of Microscopy

When

July-September 2022

Where

Yucatán Scientific Research Center (CICY) Yucatan, Mexico

program

Coming soon

2023 workshops

Fundamentals of Microscopy

When

February 2023

Where

Research Center in Optics (CIO) León, Guanajuato, Mexico

program

Coming soon

Advanced Microscopy: Laser Microdissection

When

April-June
2023

Where

Institute of Ecology, UNAM Mexico City, Mexico

Fundamentals and applications on fluorescence microscopy

When

April-June
2023

Where

LNMA-UNAM Cuernavaca, Mexico

Advanced Microscopy: Confocal, Multiphotonic, Superresolution

When

July-September
2023

Where

Neurobiology Institute, UNAM Querétaro, Mexico

Fundamentals of Microscopy

When

November 2023

Where

CICESE, Ensenada, Mexico

Advanced Microscopy: Superresolution

When

October-December
2023

Where

National Institute of Cancerology (INCAN)

program

Coming soon

2024 workshops

Fundamentals of Microscopy

When

2024

Where

Institute of Ecology (INECOL) Jalapa, Veracruz, Mexico

Image analysis: Fundamentals and applications on electron microscopy

When

January-March
2024

Where

LNMA-UNAM Cuernavaca, Mexico

Fundamentals of Microscopy

When

April-June
2024

Where

LNMA-IMSS Mexico City, Mexico

Advanced Microscopy

When

July-September
2024

Where

LNMA-UNAM Cuernavaca, Mexico

Advanced Microscopy: Superresolution and Selective Plane Illumination Microscopy (SPIM)

When

October-December
2024

Where

National Institute of Cancerology (INCAN)

if you have any questions

contact us via email at ddelgado@cicese.mx

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