Hands-on workshop on super-resolution microscopy
October 6, 2022
Welcome at LNMA-IBt
Adán Guerrero Cárdenas (LNMA-UNAM)
Instituto de Biotecnología (UNAM)
Social perception questionnaire
Roberto López
Tour at LNMA-IBt. (TIRF, Nanoimager, Confocal)
David Torres (LNMA-UNAM)
Diana Vázquez (LNMA-UNAM)
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Transfer to Hotel Montetaxco
Hands-on workshop on super-resolution microscopy – Day 1
October 7, 2022
Welcome & Opening
Adán Guerrero Cárdenas (LNMA-UNAM)
Instituto de Biotecnología (UNAM)
Fluorescence Nanoscopy Bioimaging
Mariano Buffone (IBYME-CONICET)
IBYME-CONICET
Connecting the Mexican Bioimaging Community
Diego Delgado (LNMA-CICESE)
LNMA-CICESE
Q&A
Breaking space: Mingle
Janaina García (INECOL)
Projects presentation G1
Janaina García (INECOL)
Coffee Break
From the microscope to the PSF
Miguel Tapia (Instituo de Investigaciones Biomédicas)
Super-Resolution Microscopy: opening a new era
Haydee Hernández (LNMA-UNAM)
Q & A
SIM, 4Pi, Lattice LIght Sheet, STED
Adán Guerrero Cárdenas (LNMA-UNAM)
Instituto de Biotecnología (UNAM)
Break
Scientific Talk: Super-resolution imaging reveals the structure disruption of Chromosome Territories 9 and 22 associated with treatment resistance in CML
Eunice Fabian-Morales (INCAN)
INCAN
Scientific Talk: Over-elongation of centrioles in cancer promotes centriole amplification and chromosome missegregation
Adán Guerrero Cárdenas (LNMA-UNAM)
Instituto de Biotecnología (UNAM)
Q&A
Projects presentation G2
Janaina García (INECOL)
Projects presentation G3
Janaina García (INECOL)
Q&A
Lunch
Single Molecule Localization Microscopy (STORM/PALM)
Miguel Tapia (Instituo de Investigaciones Biomédicas)
Q&A
ONI-Nanoimager (STORM 3D, SPT, smFRET)
Q&A
Hands-on workshop on super-resolution microscopy – Day 2
October 8, 2022
Virtual demo - Single Molecule Localization Microscopy
Diana Vázquez (LNMA-UNAM)
Group Photo
DNA Paint
Eduardo Brito-Alarcón (IBT – UNAM)
Spatial Statistics
David Torres (LNMA-UNAM)
Q&A
Coffee Break
Fluorescence fluctuation based super resolution microscopy
Adán Guerrero Cárdenas (LNMA-UNAM)
Instituto de Biotecnología (UNAM)
Stochastic Optical Fluctuation Imaging: theory and applications
Eduardo Brito-Alarcón (IBT – UNAM)
Q&A
Bayesian analysis of Blinking and Bleaching (3B) microscopy: theory and applications
Haydee Hernández (LNMA-UNAM)
Super Resolution Radial Fluctuation
David Torres (LNMA-UNAM)
Q&A
Round table 1
Eunice Fabian-Morales (INCAN)
INCAN
Moderator: Eunice Fabián
Q1: FF-SRM techniques are accesible and easy to use on the day-to-day basis?
Q2: How is it possible to measure or detect artifacts in FF-SRM?
Expert 1. David Torres
Expert 2. Eduardo Brito
Round table 2
Mariano Buffone (IBYME-CONICET)
IBYME-CONICET
Moderator: Mariano Buffone
Q1: What computing resources are required for SRM?
Q2: Is it possible to do SRM in 3D? Why?
Expert 3. Raul Pinto
Expert 4. Haydee Hernández
Interaction Session about the plenary
Break
Talk: Nanoscale organization of viral replication machineries
Adán Guerrero Cárdenas (LNMA-UNAM)
Instituto de Biotecnología (UNAM)
Talk: SRM on Heterologous calcium-dependent inactivation of Orai1 by neighboring TRPV1 channels
Carlos Bastián (Nikon)
Q&A
Break
Mean-Shift Super-Resolution theory
Esley Torres (CIC-UAEM)
Raúl Pinto (IICBA – UAEM)
Mean-Shift Super-Resolution applications
Adán Guerrero Cárdenas (LNMA-UNAM)
Instituto de Biotecnología (UNAM)
Q&A
Lunch
Hands-On: First steps to use MSSR
Q&A
Hands-On: Generation of SRM with MSSR
Plenary Talk: Super resolution microscopy reveals dynamic changes in the mouse sperm midpiece
Mariano Buffone (IBYME-CONICET)
IBYME-CONICET
Q&A
Hands-on workshop on super-resolution microscopy – Day 3
October 9, 2022
Hands-On: Generation of SRM with MSSR
Coffee break
Block 1: Student’s project presentations
Q&A
Block 2: Student’s project presentations
Q&A
Hands-on workshop on super-resolution microscopy – Last day
October 10, 2022
Ceremony award and closing event
Mariano Buffone (IBYME-CONICET)
IBYME-CONICET
Adán Guerrero Cárdenas (LNMA-UNAM)
Instituto de Biotecnología (UNAM)
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