Hands-on workshop on super-resolution microscopy
October 6, 2022

Welcome at LNMA-IBt

Adán Guerrero Cárdenas (LNMA-UNAM)
Instituto de Biotecnología (UNAM)
3:15 pm - 3:25 pm

Social perception questionnaire

Roberto López

Tour at LNMA-IBt. (TIRF, Nanoimager, Confocal)

David Torres (LNMA-UNAM)
Diana Vázquez (LNMA-UNAM)
5:45 pm - 6:00 pm

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6:00 pm - 8:00 pm

Transfer to Hotel Montetaxco

Hands-on workshop on super-resolution microscopy – Day 1
October 7, 2022
8:30 am - 8:35 am
Hotel Montetaxco

Welcome & Opening

Adán Guerrero Cárdenas (LNMA-UNAM)
Instituto de Biotecnología (UNAM)
8:35 am - 8:45 am
Hotel Montetaxco

Fluorescence Nanoscopy Bioimaging

Mariano Buffone (IBYME-CONICET)
IBYME-CONICET
8:45 am - 9:00 am
Hotel Montetaxco

Connecting the Mexican Bioimaging Community

Dr Diego Delgado (LNMA-CICESE)
LNMA-CICESE
9:00 am - 9:10 am
Hotel Montetaxco

Q&A

9:10 am - 9:30 am
Hotel Montetaxco

Breaking space: Mingle

Janaina García (INECOL)
9:30 am - 9:45 am
Hotel Montetaxco

Projects presentation G1

Janaina García (INECOL)
9:45 am - 10:00 am

Coffee Break

10:00 am - 10:30 am
Hotel Montetaxco

From the microscope to the PSF

Miguel Tapia (Instituo de Investigaciones Biomédicas)
10:30 am - 10:55 am
Hotel Montetaxco

Super-Resolution Microscopy: opening a new era

Haydee Hernández (LNMA-UNAM)
10:55 am - 11:00 am

Q & A

11:00 am - 11:30 am
Hotel Montetaxco

SIM, 4Pi, Lattice LIght Sheet, STED

Adán Guerrero Cárdenas (LNMA-UNAM)
Instituto de Biotecnología (UNAM)
11:30 am - 11:40 am

Break

11:40 am - 12:05 pm
Hotel Montetaxco

Scientific Talk: Super-resolution imaging reveals the structure disruption of Chromosome Territories 9 and 22 associated with treatment resistance in CML

Eunice Fabian-Morales (INCAN)
INCAN
12:05 am - 12:30 pm
Hotel Montetaxco

Scientific Talk: Over-elongation of centrioles in cancer promotes centriole amplification and chromosome missegregation

Adán Guerrero Cárdenas (LNMA-UNAM)
Instituto de Biotecnología (UNAM)
12:30 am - 12:40 pm

Q&A

12:40 pm - 1:00 pm
Hotel Montetaxco

Projects presentation G2

Janaina García (INECOL)
1:00 pm - 1:15 pm
Hotel Montetaxco

Projects presentation G3

Janaina García (INECOL)
1:15 pm - 1:30 pm

Q&A

1:30 pm - 3:00 pm

Lunch

3:00 pm - 3:30 pm
Hotel Montetaxco

Single Molecule Localization Microscopy (STORM/PALM)

Miguel Tapia (Instituo de Investigaciones Biomédicas)
3:30 pm - 3:35 pm

Q&A

3:35 pm - 4:45 pm
Hotel Montetaxco

ONI-Nanoimager (STORM 3D, SPT, smFRET)

4:45 pm - 5:00 pm

Q&A

Hands-on workshop on super-resolution microscopy – Day 2
October 8, 2022
8:30 am - 9:00 am
Hotel Montetaxco

Virtual demo - Single Molecule Localization Microscopy

Diana Vázquez (LNMA-UNAM)
9:00 am - 9:20 am
Hotel Montetaxco

Group Photo

9:20 am - 9:35 am
Hotel Montetaxco

DNA Paint

Eduardo Brito-Alarcón (IBT – UNAM)
9:35 am - 9:50 am
Hotel Montetaxco

Spatial Statistics

David Torres (LNMA-UNAM)
9:50 am - 9:55 am

Q&A

9:55 am - 10:15 am

Coffee Break

10:15 am - 10:20 am
Hotel Montetaxco

Fluorescence fluctuation based super resolution microscopy

Adán Guerrero Cárdenas (LNMA-UNAM)
Instituto de Biotecnología (UNAM)
10:20 am - 10:30 am
Hotel Montetaxco

Stochastic Optical Fluctuation Imaging: theory and applications

Eduardo Brito-Alarcón (IBT – UNAM)
10:30 am - 10:35 am

Q&A

10:35 am - 10:45 am
Hotel Montetaxco

Bayesian analysis of Blinking and Bleaching (3B) microscopy: theory and applications

Haydee Hernández (LNMA-UNAM)
10:45 am - 10:50 am
Hotel Montetaxco

Super Resolution Radial Fluctuation

David Torres (LNMA-UNAM)
10:50 am - 10:55 am

Q&A

10:55 am - 11:15 am
Hotel Montetaxco

Round table 1

Eunice Fabian-Morales (INCAN)
INCAN

Moderator: Eunice Fabián

Q1: FF-SRM techniques are accesible and easy to use on the day-to-day basis?
Q2: How is it possible to measure or detect artifacts in FF-SRM?

Expert 1. David Torres
Expert 2. Eduardo Brito

11:15 am - 11:35 am
Hotel Montetaxco

Round table 2

Mariano Buffone (IBYME-CONICET)
IBYME-CONICET

Moderator: Mariano Buffone

Q1: What computing resources are required for SRM?
Q2: Is it possible to do SRM in 3D? Why?

Expert 3. Raul Pinto
Expert 4. Haydee Hernández

11:35 am - 11:45 am
Hotel Montetaxco

Interaction Session about the plenary

11:45 am - 11:50 am

Break

11:50 am - 12:00 pm
Hotel Montetaxco

Talk: Nanoscale organization of viral replication machineries

Adán Guerrero Cárdenas (LNMA-UNAM)
Instituto de Biotecnología (UNAM)
12:00 pm - 12:25 pm
Hotel Montetaxco

Talk: SRM on Heterologous calcium-dependent inactivation of Orai1 by neighboring TRPV1 channels

Carlos Bastián (Nikon)
12:25 pm - 12:30 pm

Q&A

12:30 pm - 12:35 pm

Break

12:35 pm - 1:00 pm
Hotel Montetaxco

Mean-Shift Super-Resolution theory

Esley Torres (CIC-UAEM)
Raúl Pinto (IICBA – UAEM)
1:00 pm - 1:30 pm
Hotel Montetaxco

Mean-Shift Super-Resolution applications

Adán Guerrero Cárdenas (LNMA-UNAM)
Instituto de Biotecnología (UNAM)
1:25 pm - 1:30 pm

Q&A

1:30 pm - 3:00 pm

Lunch

3:00 pm - 3:35 pm
Hotel Montetaxco

Hands-On: First steps to use MSSR

3:35 pm - 3:40 pm

Q&A

3:40 pm - 4:05 pm

Hands-On: Generation of SRM with MSSR

4:05 pm - 4:45 pm
Hotel Montetaxco

Plenary Talk: Super resolution microscopy reveals dynamic changes in the mouse sperm midpiece

Mariano Buffone (IBYME-CONICET)
IBYME-CONICET
4:45 pm - 5:00 pm

Q&A

Hands-on workshop on super-resolution microscopy – Day 3
October 9, 2022
8:30 am - 11:30 am
Hotel Montetaxco

Hands-On: Generation of SRM with MSSR

11:30 am - 11:45 am

Coffee break

11:45 am - 12:35 pm
Hotel Montetaxco

Block 1: Student’s project presentations

12:35 pm - 12:45 pm

Q&A

12:45 pm - 1:20 pm

Block 2: Student’s project presentations

1:20 pm - 1:30 pm

Q&A

Hands-on workshop on super-resolution microscopy – Last day
October 10, 2022

Ceremony award and closing event

Mariano Buffone (IBYME-CONICET)
IBYME-CONICET
Adán Guerrero Cárdenas (LNMA-UNAM)
Instituto de Biotecnología (UNAM)
10:00 am - 10:30 am
Hotel Montetaxco

Final thoughts and feedback from assistants

11:30 am - 1:30 pm

Transfer to LNMA-IBt

if you have any questions

contact us via email at ddelgado@cicese.mx

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