The microscopy basis represents the theoretical bases for the correct use of this powerful tool, which supports research projects ranging from physics, life sciences, chemistry to materials. Through microscopy it is possible to get images as evidence of experiments. This is why students, technicians and researchers that use microscopes need to understand the basic physics of microscopy, for conducting towards more advanced and sophisticated microscopy techniques such as confocal microscopy, electronic microscopy, super resolution and laser microdissection. The main objective of this course is to provide an introduction to the theoretical bases of microscopy to undergraduate and graduate students, technicians and researchers with minimum experience in microscopy. Through this workshop we aim to promote the use of optical, electronic and tunneling microscopy in the center region of Mexico. At the Institute of Ecology and Cell Physiology Institute, we have microscopy facilities that will be available during the course to students and researchers. The general goal is to consolidate the Bioimaging community in México as well as in the center region, in the framework of the Latin-Bioimaging initiatives and the CZI project “Expanding Global Access to Bioimaging”.
MBW5: Microscopía Óptica y Electrónica-Técnicas avanzadas y Microdisección a láser – Day 1
June 13, 2023

Welcome and opening

Ana Elena Escalante Hernández (Institute of Ecology, UNAM)
8:40 am - 8:50 am

Connecting the Mexican Bioimaging Community

Diego Delgado (LNMA-CICESE)
LNMA-CICESE

Social perception questionnaire

Adán Guerrero Cárdenas (LNMA-UNAM)
Instituto de Biotecnología (UNAM)
9:00 am - 9:30 am

A Brief Historical Outline of Microscopy

Gastón Contreras Jiménez
Instituto de Ecología, UNAM

Properties and Behavior of Light

Alejandro Vázquez Arzola (Institute of Physics, UNAM)

Light Sources and propagation through the media: Interaction of Light with Matter

Alejandro Vázquez Arzola (Institute of Physics, UNAM)
10:30 am - 10:45 am

Q & A

10:45 am - 11:00 am

Coffee -break

Optical Detectors (Camera types: CCD, CMOS)

Jaime Arturo Pimentel Cabrera (Institute of Biotechnology, UNAM)

Digital Images

José Manuel Martínez López (Quimica TECH)

José Manuel Martínez López (Quimica TECH)
12:45 am - 1:00 pm

Q & A

1:00 pm - 2:00 pm

Connecting the community / Icebreaker2

Presentations for 3min each assistant about their project, expectations for their careers, hobbies and interests.

2:00 pm - 3:30 pm

Lunch

DEMO1: Light Behavior

Alejandro Vázquez Arzola (Institute of Physics, UNAM)
4:30 pm - 5:00 pm

DEMO: The Human Eye

Museo de la Luz, DGDC-UNAM

DEMO2: Stereoscopic visualization

Fernando Guerrero Aguilar (KEYGENCE México S. A. de C. V.)
6:00 pm - 6:15 pm

Opening Group Photo (Presencial)

MBW5: Microscopía Óptica y Electrónica-Técnicas avanzadas y Microdisección a láser – Day 2
June 14, 2023
8:45 am - 9:00 am

BioImaging North America program

Nikki Bialy

Brightfield and Darkfield Microscopy

José Manuel Martínez López (Quimica TECH)

Phases Contrast Microscopy and Differential Interference Contrast Microscopy

Andrés Saralegui (Institute of Biotechnology)

Polarized Light Microscopy

José Manuel Martínez López (Quimica TECH)
10:30 am - 11:00 am

Fluorescence and Widefield Fluorescence Microscopy

Miguel Tapia
Instituto de Investigaciones Biomédicas
11:00 am - 11:15 am

Q & A

11:15 am - 11:30 am

Coffee Break

Total Internal Reflection Fluorescence Microscopy and HILO

Luis Vaca Domínguez (Cell Physiology Institute, UNAM)

Laser Scanning and Spinning Disc Confocal Microscopy

Iván Galván Mendoza (Center for Research and Advanced Studies, IPN)

Analysis and Processing of Images

Jaime Arturo Pimentel Cabrera (Institute of Biotechnology, UNAM)
1:00 pm - 1:15 pm

Q & A

1:15 pm - 1:30 pm

Stereoscopic Imaging and rendering for insect reconstruction

Armando Burgos Solorio (Center for Biological Research, UAEMor)
2:30 pm - 4:00 pm

Lunch

DEMO: Confocal Cytation10

Yolanda Ríos (Agilent México S. A. de C. V.)

DEMO: Practical Microscopy

José Manuel Martínez López (Quimica TECH)
MBW5: Microscopía Óptica y Electrónica-Técnicas avanzadas y Microdisección a láser – Day 3
June 15, 2023
8:45 am - 9:00 am

Latin America Bioimaging (virtual)

Gastón Contreras Jiménez
Instituto de Ecología, UNAM
9:00 am - 9:20 am

Sample Preparation for Optical microscopy

Ruth Rincón Heredia
Instituto de Fisiología Celular (UNAM).

Introduction to Histology: Animal Tissues

Sandra Daniela Rodríguez Montaño (Institute of Cell Physiology, UNAM)

Introduction to Histology: Plant Tissues

Estela Sandoval Zapotitla (Botanic Garden, Institute of Biology, UNAM)
10:20 am - 10:30 am

Q & A

10:30 am - 10:40 am

Coffee Break

10:40 am - 11:00 am

Introduction to Laser Microdissection Technique

Gastón Contreras Jiménez
Instituto de Ecología, UNAM

Laser Microdissection to Induce DNA damage in Live Cells

Diego Oliva Rico (National Institute of Cancerology)

Laser Microdissection for Embryology and Cancer Applications

Enrique Antonio Pedernera Astegiano (Faculty of Medicine, UNAM)

Laser Microdissection General Applications

Pablo Ernesto Pomata (Biology Institute & Experimental Medicine CONICET, ARG)
1:00 pm - 1:10 pm

Q & A

1:10 pm - 1:20 pm

Coffee Break

1:20 pm - 2:20 pm

Artificial Intelligence applied to Confocal Microscopy (Virtual)

Mónica Roldán Molina
Hospital San Juan de Dios de Barcelona
2:20 pm - 3:30 pm

Lunch

DEMO1: Plant Histology

Estela Sandoval Zapotitla (Botanic Garden, Institute of Biology, UNAM)
4:15 pm - 5:00 pm

DEMO2: Laser Microdissection

Gastón Contreras Jiménez
Instituto de Ecología, UNAM

DEMO3: Animal Histology

Sandra Daniela Rodríguez Montaño (Institute of Cell Physiology, UNAM)
5:45 pm - 6:30 pm

DEMO4: Confocal Microscopy

Miguel Tapia
Instituto de Investigaciones Biomédicas
MBW5: Microscopía Óptica y Electrónica-Técnicas avanzadas y Microdisección a láser – Day 4
June 16, 2023

Multiphoton Confocal Microscopy and Applications

Yazmín Ramiro Cortés (Institute of Cell Physiology, UNAM)
9:00 am - 9:30 am

Super-Resolution Microscopy and Applications

Carlos Bastián (Nikon)

Scanning Electron Microscopy and Applications

María Berenit Mendoza Garfías (Institute of Biology, UNAM)
10:00 am - 10:15 am

Q & A

10:15 am - 10:30 am

Coffee Break

Transmission Electron Microscopy and Applications

Ana Paulina Mendoza Von der Borch (Faculty of Sciences, UNAM)

Atomic Force Microscopy and Applications

Armando Hernández García (Institute of Chemistry, UNAM)

Scanning Tunneling Microscopy and Applications

Carlos Javier Villagómez Ojeda (Institute of Physics, UNAM)

Optical & Electron Correlation Microscopy

David Mauricio Giraldo Gómez (Zeiss México S. A. de C. V.)
12:30 pm - 12:45 pm

Q & A

12:45 pm - 1:00 pm

Social perception questionnaire

Adán Guerrero Cárdenas (LNMA-UNAM)
Instituto de Biotecnología (UNAM)
1:00 pm - 2:00 pm

Discovering Nucleolus inside Procariota

Luis Felipe Jiménez García
Facultad de Ciencias (UNAM)

Closure

María Soledad Funes Argüello, Director at Cell Physiology Institute, UNAM
2:20 pm - 3:30 pm

Lunch

3:30 pm - 4:15 pm

DEMO1: Atomic Force Microscopy

4:15 pm - 5:00 pm

DEMO2: Scanning Electron Microscopy

5:00 pm - 5:45 pm

DEMO3: Transmission Electron Microscopy

5:45 pm - 6:30 pm

DEMO4: Scanning Tunneling Microscopy

6:30 pm - 7:00 pm

Closing - Group Photo

if you have any questions

contact us via email at meximaging@gmail.com or ddelgado@cicese.mx

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