The microscopy basis represents the theoretical bases for the correct use of this powerful tool, which supports research projects ranging from physics, life sciences, chemistry to materials. Through microscopy it is possible to get images as evidence of experiments. This is why students, technicians and researchers that use microscopes need to understand the basic physics of microscopy, for conducting towards more advanced and sophisticated microscopy techniques such as confocal microscopy, electronic microscopy, super resolution and laser microdissection. The main objective of this course is to provide an introduction to the theoretical bases of microscopy to undergraduate and graduate students, technicians and researchers with minimum experience in microscopy. Through this workshop we aim to promote the use of optical, electronic and tunneling microscopy in the center region of Mexico. At the Institute of Ecology and Cell Physiology Institute, we have microscopy facilities that will be available during the course to students and researchers. The general goal is to consolidate the Bioimaging community in México as well as in the center region, in the framework of the Latin-Bioimaging initiatives and the CZI project “Expanding Global Access to Bioimaging”.
MBW5: Microscopía Óptica y Electrónica-Técnicas avanzadas y Microdisección a láser – Day 1
June 13, 2023
Welcome and opening
Ana Elena Escalante Hernández (Institute of Ecology, UNAM)
Connecting the Mexican Bioimaging Community
Diego Delgado (LNMA-CICESE)
LNMA-CICESE
Social perception questionnaire
Adán Guerrero Cárdenas (LNMA-UNAM)
Instituto de Biotecnología (UNAM)
A Brief Historical Outline of Microscopy
Gastón Contreras Jiménez
Instituto de Ecología, UNAM
Properties and Behavior of Light
Alejandro Vázquez Arzola (Institute of Physics, UNAM)
Light Sources and propagation through the media: Interaction of Light with Matter
Alejandro Vázquez Arzola (Institute of Physics, UNAM)
Q & A
Coffee -break
Optical Detectors (Camera types: CCD, CMOS)
Jaime Arturo Pimentel Cabrera (Institute of Biotechnology, UNAM)
Digital Images
José Manuel Martínez López (Quimica TECH)
José Manuel Martínez López (Quimica TECH)
Q & A
Connecting the community / Icebreaker2
Presentations for 3min each assistant about their project, expectations for their careers, hobbies and interests.
Lunch
DEMO1: Light Behavior
Alejandro Vázquez Arzola (Institute of Physics, UNAM)
DEMO: The Human Eye
Museo de la Luz, DGDC-UNAM
DEMO2: Stereoscopic visualization
Fernando Guerrero Aguilar (KEYGENCE México S. A. de C. V.)
Opening Group Photo (Presencial)
MBW5: Microscopía Óptica y Electrónica-Técnicas avanzadas y Microdisección a láser – Day 2
June 14, 2023
BioImaging North America program
Nikki Bialy
Brightfield and Darkfield Microscopy
José Manuel Martínez López (Quimica TECH)
Phases Contrast Microscopy and Differential Interference Contrast Microscopy
Andrés Saralegui (Institute of Biotechnology)
Polarized Light Microscopy
José Manuel Martínez López (Quimica TECH)
Fluorescence and Widefield Fluorescence Microscopy
Miguel Tapia
Instituto de Investigaciones Biomédicas
Q & A
Coffee Break
Total Internal Reflection Fluorescence Microscopy and HILO
Luis Vaca Domínguez (Cell Physiology Institute, UNAM)
Laser Scanning and Spinning Disc Confocal Microscopy
Iván Galván Mendoza (Center for Research and Advanced Studies, IPN)
Analysis and Processing of Images
Jaime Arturo Pimentel Cabrera (Institute of Biotechnology, UNAM)
Q & A
Stereoscopic Imaging and rendering for insect reconstruction
Armando Burgos Solorio (Center for Biological Research, UAEMor)
Lunch
DEMO: Confocal Cytation10
Yolanda Ríos (Agilent México S. A. de C. V.)
DEMO: Practical Microscopy
José Manuel Martínez López (Quimica TECH)
MBW5: Microscopía Óptica y Electrónica-Técnicas avanzadas y Microdisección a láser – Day 3
June 15, 2023
Latin America Bioimaging (virtual)
Gastón Contreras Jiménez
Instituto de Ecología, UNAM
Sample Preparation for Optical microscopy
Ruth Rincón Heredia
Instituto de Fisiología Celular (UNAM).
Introduction to Histology: Animal Tissues
Sandra Daniela Rodríguez Montaño (Institute of Cell Physiology, UNAM)
Introduction to Histology: Plant Tissues
Estela Sandoval Zapotitla (Botanic Garden, Institute of Biology, UNAM)
Q & A
Coffee Break
Introduction to Laser Microdissection Technique
Gastón Contreras Jiménez
Instituto de Ecología, UNAM
Laser Microdissection to Induce DNA damage in Live Cells
Diego Oliva Rico (National Institute of Cancerology)
Laser Microdissection for Embryology and Cancer Applications
Enrique Antonio Pedernera Astegiano (Faculty of Medicine, UNAM)
Laser Microdissection General Applications
Pablo Ernesto Pomata (Biology Institute & Experimental Medicine CONICET, ARG)
Q & A
Coffee Break
Artificial Intelligence applied to Confocal Microscopy (Virtual)
Mónica Roldán Molina
Hospital San Juan de Dios de Barcelona
Lunch
DEMO1: Plant Histology
Estela Sandoval Zapotitla (Botanic Garden, Institute of Biology, UNAM)
DEMO2: Laser Microdissection
Gastón Contreras Jiménez
Instituto de Ecología, UNAM
DEMO3: Animal Histology
Sandra Daniela Rodríguez Montaño (Institute of Cell Physiology, UNAM)
DEMO4: Confocal Microscopy
Miguel Tapia
Instituto de Investigaciones Biomédicas
MBW5: Microscopía Óptica y Electrónica-Técnicas avanzadas y Microdisección a láser – Day 4
June 16, 2023
Multiphoton Confocal Microscopy and Applications
Yazmín Ramiro Cortés (Institute of Cell Physiology, UNAM)
Super-Resolution Microscopy and Applications
Carlos Bastián (Nikon)
Scanning Electron Microscopy and Applications
María Berenit Mendoza Garfías (Institute of Biology, UNAM)
Q & A
Coffee Break
Transmission Electron Microscopy and Applications
Ana Paulina Mendoza Von der Borch (Faculty of Sciences, UNAM)
Atomic Force Microscopy and Applications
Armando Hernández García (Institute of Chemistry, UNAM)
Scanning Tunneling Microscopy and Applications
Carlos Javier Villagómez Ojeda (Institute of Physics, UNAM)
Optical & Electron Correlation Microscopy
David Mauricio Giraldo Gómez (Zeiss México S. A. de C. V.)
Q & A
Social perception questionnaire
Adán Guerrero Cárdenas (LNMA-UNAM)
Instituto de Biotecnología (UNAM)
Discovering Nucleolus inside Procariota
Luis Felipe Jiménez García
Facultad de Ciencias (UNAM)
Closure
María Soledad Funes Argüello, Director at Cell Physiology Institute, UNAM
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