Summary
The generation of scientific knowledge about ecosystems, biological diversity and the understanding of biological processes at the cellular level from a multidisciplinary perspective have contributed to the solution of environmental, agricultural, forestry and health problems for the benefit of society. The 10th Edition of the Mexican Bioimaging Workshops, is focused on the use of advanced microscopy tools (Confocal, SEM and TEM) in the processing and study of biological samples such as microorganisms, plants and insects that allow the study of organisms from an ecological perspective. systematic. , biochemistry and cellular in order to understand the molecular mechanisms of biological processes. We will address fundamentals and applications of fluorescence microscopy such as epifluorescence, confocal and super resolution (STED), sample preparation and visualization using scanning electron microscopy (SEM and SEM-EDS) and transmission, as well as sample preparation techniques such as cryosubstitution, cryofixation, microcomputed tomography (Micro-CT) among other applications.
Resumen
La generación de conocimiento científico sobre los ecosistemas, la diversidad biológica hasta el entendimiento de los procesos biológicos a nivel celular desde una perspectiva multidisciplinaria, han contribuido a la solución de problemas ambientales, agrícolas, forestales y salud en beneficio de la sociedad. En esta 10º. Edición de los Mexican Bioimaging Workshops, está enfocada en el uso herramientas de microscopía avanzada (Confocal, SEM y TEM) en el procesamiento y estudio de muestras biológicas como microorganismos, plantas e insectos que permitan su estudio de los organismos desde una perspectiva ecológica, sistémica, bioquímica y celular en aras de entender los mecanismos moleculares de los procesos biológicos. Abordaremos fundamentos y aplicaciones de la microscopía de fluorescencia como epifluorescencia, confocal y de super resolución (STED), la preparación de muestras y visualización mediante microscopía electrónica de barrido (SEM y SEM-EDS) y transmisión, así como técnicas de preparación de muestras como de criosustitución, criofijación, microtomografía computarizada (Micro-CT) entre otras aplicaciones.
Fluorescence microscopy: Epifluorescence, Confocal and STED
Departure from Hotel
Registration
Welcome and opening
Connecting the Mexican Bioimaging Community
Coffee break and Icebreaker
Introduction to Fluorescence and Confocal Microscopy
Laser Scanning and Spinning Disk Confocal Microscopy
Introduction to STED Analysis and Applications
Image processing and analysis
Q&A
Meal
Group photo
Practical Session (Ángela Francisca Kú González, CICY, Piero Bagnaresi, Leica, Olinda Elizabeth Velázquez López, INECOL)
Sample Preparation for Epifluorescence, Confocal and Super-resolution Microscopy STED Microscopy Practice
Back to Hotel
Moderator: Randy Ortíz Castro (INECOL)
Scanning Electron Microscopy, Technique Usage and Applications
Departure from Hotel
Registration
Welcome and opening
Connecting the Mexican Bioimaging Community
Coffee break and Icebreaker
Introduction to Fluorescence and Confocal Microscopy
Laser Scanning and Spinning Disk Confocal Microscopy
Introduction to STED Analysis and Applications
Image processing and analysis
Q&A
Meal
Group photo
Practical Session (Ángela Francisca Kú González, CICY, Piero Bagnaresi, Leica, Olinda Elizabeth Velázquez López, INECOL)
Sample Preparation for Epifluorescence, Confocal and Super-resolution Microscopy
STED Microscopy Practice
Back to Hotel
Moderator: Lorena María Luisa López Sánchez (INECOL)
Cryosubstitution, Cryofixation, Computed Microtomography and Applications in Biological Samples
Departure from Hotel
Latin America Bioimaging
Fundamentals of Transmission Electron Microscopy (TEM)
Coffee Break
Applications of Transmission Electron Microscopy in Biological Samples.
Cryo Electron Microscopy
Correlative fluorescence and Electron Microscopy for the Localization of Extracellular Vesicles and Viral Particles
Q&A
Meal
Practical Session (Lorena María Luisa López Sánchez, INECOL, Luis Felipe Jiménez García, Ciencias, UNAM)
Using TEM and Preparing Samples for TEM
Using the Ultramicrotome and Sections
Back to Hotel
Moderator: Olinda Elizabeth Velázquez López (INECOL)
Transmission Electron Microscopy, Technique Usage and Applications
Departure from Hotel
Global Bioimaging (GBI)
Theory and Applications of Cryofixation
Theory and Applications of Cryosubstitution
Q&A
Coffee Break
Volume Electron Microscopy
Computed Microtomography (MicroCT)
Introduction and applications: Atomic force
Q&A
Meal
Exhibition of Suppliers of Microscopy Equipment and Applications in Biological Samples
Group Photo
Social Appropriation of Knowledge: Diego Luis Delgado Álvarez (LNMA-CICESE) y Juan Arturo Piña Martínez (INECOL)
Place: Botanical Garden “Francisco Javier Clavijero”
Closing of the 10th Mexican Bioimaging Workshops
Back to Hotel
Dinner
Moderator: Randy Ortíz Castro (INECOL)
Departure from Hotel
Getting to know the Organisms of the Fog Forest: Workshop and Exhibition of Micrographs of Biological Samples from the Advanced Microscopy Unit (UMA-INECOL)
Mexican Bioimaging Workshops 10 Facilitators
Place: Botanical Garden “Francisco Javier Clavijero”
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